531-302 Techniques for Investigation of Disease
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Students intending to complete a major in pathology are required to enrol in both 531-301 and 531-302.
To enrol in 531-303 and 531-304, a pass must be achieved in both 531-301 and 531-302. Credit cannot be gained for 531-302 and 531-301 prior to 2000.
Number of points: 12.5
Subject co-ordinators: Dr J R Underwood; Dr M M Ayers
Pre-requisites:
531-201; biochemistry and molecular biology 521-211, 521-212 and 521-220.
The following subjects are recommended: anatomy and cell biology 516-201; biochemistry and molecular biology 521-301 and/or 521-302; or microbiology and immunology 526-304 plus 526-324.
531-301 Cellular Basis of Disease.
Contact:
A maximum of 54 hours of practical work comprising six 6-hour laboratory-based practicals and six 3-hour written data-exercises directly related to the laboratory work
Students completing this subject will:
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gain a deeper theoretical and practical understanding of the way in which questions about disease processes are formulated and investigation of these questions is carried out using sophisticated laboratory-based techniques; and
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take part in hands-on laboratory experiments using current techniques (see below) appropriate for investigation of a variety of diseases; also complete written exercises based on interpretation of unseen experimental data.
The techniques to be studied are immunofluorescence and immunocytochemistry, ELISA, immunoblotting and molecular biology techniques.
Assessment:
Practical work (65%). Practical reports are to be submitted weekly in accordance with the practical submission timetable provided in the subject handbook. Continuous assessment of laboratory performance (10%); A 1-hour end-of-semester written examination (25%). Attendance at all pre-practical talks and all practical sessions as indicated in the subject practical manual is compulsory. Completion and submission of all practical reports by the submission dates indicated in the subject practical manual is compulsory.
Prescribed texts:
A laboratory manual and references to current scientific journal articles will be available at the beginning of the semester.